Ctla4 Recombinant Antibody
Western Blot; Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, MCF7 whole cell lysate; All lanes: CTLA4 antibody at 1:500; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 25 kDa; Observed band size: 25 kDa
Immunofluorescence staining of HEPG2 cell with RAC07856 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay Peak curve showing JK cells stained with RAC07856 (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,015 events was performed.
Ctla4 Recombinant Antibody
Western Blot; Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, MCF7 whole cell lysate; All lanes: CTLA4 antibody at 1:500; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 25 kDa; Observed band size: 25 kDa
Immunofluorescence staining of HEPG2 cell with RAC07856 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay Peak curve showing JK cells stained with RAC07856 (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,015 events was performed.

Ctla4 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07856-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P16410
Applications
ELISA, WB, IF, FC
Buffer
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Clonality
Recombinant Monoclonal
Clone number
13D6
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Ctla4 Recombinant Antibody is applicable for the following applications: ELISA, WB, IF, FC.
Expiration
12 months
Format
Liquid
Host
Mouse
Immunogen
Recombinant Human Ctla4 Protein
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Immunology, Stem Cells
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
CTLA4
Target synonyms
ALPS5; CD 152; CD; CD152; CD152 antigen; CD152 isoform; Celiac disease 3; CELIAC3; CTLA 4; CTLA-4; CTLA4; CTLA4_HUMAN; Cytotoxic T cell associated 4; Cytotoxic T lymphocyte antigen 4; Cytotoxic T lymphocyte associated 4; Cytotoxic T lymphocyte associated 4; soluble isoform; included; Cytotoxic T lymphocyte associated antigen 4; Cytotoxic T lymphocyte associated antigen 4 short spliced form; Cytotoxic T lymphocyte associated protein 4; Cytotoxic T lymphocyte associated serine esterase 4; Cytotoxic T lymphocyte protein 4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; GRD4; GSE; ICOS; IDDM12; insulin-dependent diabetes mellitus 12; Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; OTTHUMP00000216623
Ctla4 Recombinant Antibody

Ctla4 Recombinant Antibody

$575.00 USD