Hist1H3A Recombinant Antibody
Western Blot; Positive WB detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; All lanes: HIST1H3A antibody at 1:1000; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 16 kDa; Observed band size: 16 kDa
IHC image of RAC07886 diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with RAC07886 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Hist1H3A Recombinant Antibody
Western Blot; Positive WB detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; All lanes: HIST1H3A antibody at 1:1000; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 16 kDa; Observed band size: 16 kDa
IHC image of RAC07886 diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with RAC07886 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Hist1H3A Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07886-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P68431
Applications
ELISA, WB, IHC, IF
Buffer
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Clonality
Recombinant Monoclonal
Clone number
9G8
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Hist1H3A Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Mouse
Ihc validation
IHC Validated
Immunogen
Recombinant Human Hist1H3A Protein
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HIST1H3A
Target synonyms
H3 histone family member E pseudogene; H3 histone family; member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1; H3a; Histone cluster 1; H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Hist1H3A Recombinant Antibody

Hist1H3A Recombinant Antibody

$575.00 USD