ICAM1 Recombinant Monoclonal Antibody
Western Blot. Positive WB detected in:K562 whole cell lysate (20µg), A375 whole cell lysate (20µg). All lanes: ICAM1 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:57.83 kDa. Observed band size:58 kDa. Exposure time:120s
IHC image diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing HepG2 cells stained at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was human IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
ICAM1 Recombinant Monoclonal Antibody
Western Blot. Positive WB detected in:K562 whole cell lysate (20µg), A375 whole cell lysate (20µg). All lanes: ICAM1 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:57.83 kDa. Observed band size:58 kDa. Exposure time:120s
IHC image diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing HepG2 cells stained at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was human IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ICAM1 Recombinant Monoclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC08080-100UL

$575.00 USD
Shipping calculated at checkout.

Available in stock (500)

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  • Worldwide shipping available.
For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P05362
Applications
ELISA, WB, IHC, FC
Buffer
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Clonality
Recombinant Monoclonal
Clone number
15A9
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The ICAM1 Recombinant Monoclonal Antibody is applicable for the following applications: ELISA, WB, IHC, FC.
Expiration
12 months
Format
Liquid
Host
Human
Ihc validation
IHC Validated
Immunogen
Recombinant Human ICAM1 protein
Isotype
IgG1
Lead time for package collection
7-11 Business Days
Purification
Affinity chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cardiovascular, Immunology, Stem cells
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
ICAM1
Target synonyms
Antigen identified by monoclonal antibody BB2 antibody; BB 2 antibody; BB2 antibody; CD 54 antibody; CD_antigen=CD54 antibody; CD54 antibody; Cell surface glycoprotein P3.58 antibody; Human rhinovirus receptor antibody; ICAM 1 antibody; ICAM-1 antibody; ICAM1 antibody; ICAM1_HUMAN antibody; intercellular adhesion molecule 1 (CD54) antibody; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody; Intercellular adhesion molecule 1 antibody; Major group rhinovirus receptor antibody; MALA 2 antibody; MALA2 antibody; MyD 10 antibody; MyD10 antibody; P3.58 antibody; Surface antigen of activated B cells antibody; Surface antigen of activated B cells, BB2 antibody
ICAM1 Recombinant Monoclonal Antibody

ICAM1 Recombinant Monoclonal Antibody

$575.00 USD