Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit

Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK2629

$458.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Enzyme & Kinase;Neuro science;
Sample type
Tissue homogenates and other biological fluids.
Sensitivity
0.046 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q5EZ72
Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit

Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit

$458.00 USD