Rat COX1(Cytochrome C Oxidase Subunit I) ELISA Kit
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$458.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
Product Overview
Product Overview
Assay length
3.5h
Assay type
Sandwich
Detection range
0.32-20 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Enzyme & Kinase;
Sample type
serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.11 ng/mL
Standard
20 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COX1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P05503
Background
Background
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More Info
More Info
Rat COX1(Cytochrome C Oxidase Subunit I) ELISA Kit
Regular price
$458.00 USD
Sale price
Regular price
Unit price
/per
