The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.

Q8R414

around 3 working days

L 16cm x W 15cm x H 9cm

Cytokine;Tumor immunity;

3.5h

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

15.63-1000 pg/mL

1000 pg/mL

6.4 pg/mL

Sandwich

Rat