Rat HGFAC(Hepatocyte Growth Factor Activator) ELISA Kit

Item number: ELK7372
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
Please hurry! Only 100 left in stock

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Size: 48T
Subtotal: $320.00
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Rat HGFAC(Hepatocyte Growth Factor Activator) ELISA Kit

Rat HGFAC(Hepatocyte Growth Factor Activator) ELISA Kit

$320.00

Rat HGFAC(Hepatocyte Growth Factor Activator) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HGFAC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HGFAC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HGFAC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HGFAC in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q5EBA7
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Immune molecule;
Assay length
3.5h
Sample type
Serum, plasma, tissue homogenates and other biological fluids.
Detection range
15.63-1000 pg/mL
Standard
1000 pg/mL
Sensitivity
5.7 pg/mL
Assay type
Sandwich
Reactivity
Rat

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