Rat HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
Rat HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Rat HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK1604

$458.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Tumor immunity;Cardiovascular biology;Reproductive science;
Sample type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.054 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HIF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HIF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HIF1a in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
O35800
Rat HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Rat HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

$458.00 USD