Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit

Item number: ELK7160
Availability: In Stock
Product Type: Precoated
$320.00
$320.00
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Size: 48T
Subtotal: $320.00
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Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit

Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit

$320.00

Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit

$320.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MGMT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MGMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MGMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MGMT in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P24528
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;Enzyme & Kinase;Tumor immunity;
Assay length
3.5h
Sample type
Tissue homogenates and other biological fluids.
Detection range
78.13-5000 pg/mL
Standard
5000 pg/mL
Sensitivity
27 pg/mL
Assay type
Sandwich
Reactivity
Rat

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