Rat PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit

Name: Rat PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit
Brand: ELK Biotechnology
Price: 458.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK0304

$458.00 USD

Shipping calculated at checkout.

Documents

Manuals

SDS

Supplier image

Available in stock (100)

Customs clearance fees may apply
Worldwide shipping available.

For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

0.16-10 ng/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Rat

Research area

Reproductive science;Genetic science;

Sample type

serum, plasma, tissue homogenates and other biological fluids

Sensitivity

0.05 ng/mL

Standard

10 ng/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAPPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAPPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAPPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAPPA in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit

Documents

Product Overview

Background

Documents

Documents

Product Citations

More Info