Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

Name: Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit
Brand: ELK Biotechnology
Price: 458.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK10655

$458.00 USD

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

0.16-10 ng/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Rat

Research area

Enzyme & Kinase;Metabolic pathway;Endocrinology;Hepatology;Nutrition metabolism;

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

0.068 ng/mL

Standard

10 ng/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SOD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SOD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SOD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

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