Rat TFAM(Transcription Factor A, Mitochondrial) ELISA Kit
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$458.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
Product Overview
Product Overview
Assay length
3.5h
Assay type
Sandwich
Detection range
0.32-20 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Signal transduction;
Sample type
serum, plasma and other biological fluids
Sensitivity
0.11 ng/mL
Standard
20 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFAM in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q91ZW1
Background
Background
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More Info
More Info
Rat TFAM(Transcription Factor A, Mitochondrial) ELISA Kit
Regular price
$458.00 USD
Sale price
Regular price
$0.00 USD
Unit price
/per
