Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit
Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit

Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8353

$320.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
2h
Assay type
Sandwich
Detection range
1.57-100 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Rat
Research area
Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Sample type
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.62 ng/mL
Standard
100 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TG-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TG-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TG-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TG-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit

Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit

$320.00 USD