This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRH in the samples is then determined by comparing the OD of the samples to the standard curve.

P01150

around 3 working days

L 16cm x W 15cm x H 9cm

Endocrinology;Neuro science;Hepatology;Hormone metabolism;

2h

serum, plasma and other biological fluids

7.82-500 pg/mL

500 pg/mL

2.52 pg/mL

Competitive Inhibition

Rat