Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

Item number: EPT003
Availability: In Stock
$839.00
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Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

$839.00

Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

$839.00
Size: 50ug
Description
Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.
Accession
YP_009725309.1
Molecular weight
62.9 KDa
Apparent molecular weight
60 KDa, reducing conditions
Other names
SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
Purity
Greater than 85% as determined by reducing SDS-PAGE.
Storage
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Delivery condition
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

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