Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP325199BUW

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Bothrops jararaca Snake venom metalloproteinase (SVMP) protein, partial
Alternative names
Snake venom metalloproteinase von Willebrand factor-dependent platelet coagglutinin
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
1-25aa
Form
Liquid or Lyophilized powder
Mw
18.0 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Bothrops jararaca (Jararaca) (Bothrops jajaraca)
Protein length
Partial
Purity
Greater than 85% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"Isolation and chemical characterization of two structurally and functionally distinct forms of botrocetin, the platelet coagglutinin isolated from the venom of Bothrops jararaca." Fujimura Y., Titani K., Usami Y., Suzuki M., Oyama R., Matsui T., Fukui H., Sugimoto M., Ruggeri Z.M. Biochemistry 30:1957-1964(1991)
Relevance
Metalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation. Miscellaneous There are two distinct forms of the vWF-dependent platelet coagglutinin. The dimeric form (snaclec) is 34-fold more active than the metalloprotease botrocetin in promoting vWF binding to platelets (PubMed:1993206).
Research area
others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 6xHis-KSI-tagged
Target protein sequenc
IISPPVCGNELLEEGEECDCGTPEN
Uniprot id
P22028
Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial

Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial

$2,466.00 USD