Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP451740BXS

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Burkholderia cenocepacia lpxC protein
Alternative names
UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
1-305aa
Form
Liquid or Lyophilized powder
Function
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.
Gene names
lpxC
Mw
51 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Protein length
Full Length
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients." Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D., Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S., Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K., Scott P., Walker D. Parkhill J. J. Bacteriol. 191:261-277(2009)
Relevance
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 6xHis-SUMO-tagged and C-terminal Myc-tagged
Target protein sequenc
MLKQRTIKSIVKTVGIGVHSGRKIELTLRPAAPGTGIVFSRVDLPTPVDIPASAMSIGDTRLASVLQKDGVRVSTVEHLMSACAGLGIDNLYVDVTAEEIPIMDGSAATFVFLIQSAGIEEQNAPKRFIKVKKPVEIRDGDKFARLDPYFGFKLKFSIDFRHPAVDKTGQELEVDFATTSYVREIARARTFGFAHEAEMLREIGLARGGSMDNAIVLDEYRILNNDGLRYDDEFVKHKMLDAIGDLYVIGHPLLASYTAYKSGHGLNNALLRELLAHEDAYEIVTFDDPQAAPKGFAFDAQTAFA
Uniprot id
B4E5Y5
Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)

Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)

$2,466.00 USD