Recombinant Dendroaspis angusticeps Fasciculin-2 (Fas-2)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP314272DBG

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Dendroaspis angusticeps Fas-2 protein
Alternative names
Short name:Fas-2 Short name:Fas2 Alternative name(s): Acetylcholinesterase toxin F-VII Fasciculin-II Short name:FAS-II Toxin TA1
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
1-61aa
Form
Liquid or Lyophilized powder
Function
Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1
Gene names
Fas-2
Mw
22.8 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Protein length
Full Length
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"Snake venom toxins. The purification and amino acid sequence of toxin F-VII from Dendroaspis angusticeps venom."Viljoen C.C., Botes D.P.J. Biol. Chem. 248:4915-4919(1973)
Relevance
Interferes with neuromuscular transmission by inhibiting the enzyme acetylcholinesterase (AChE) present at the neuromuscular junction. It selectively binds and inhibits with a 1:1 stoichiometry the mammalian and electric fish AChE at picomolar concentrations. It is highly specific for the peripheral site of AChE and blocks the entry of acetylcholine into the active site of the enzyme (through the Met-33 residue), thereby preventing its breakdown. It has been called fasciculin since after injection into mice it causes severe, generalized and long-lasting (5-7 hours) fasciculations.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 6xHis-SUMO-tagged
Target protein sequenc
TMCYSHTTTSRAILTNCGENSCYRKSRRHPPKMVLGRGCGCPPGDDNLEVKCCTSPDKCNY
Uniprot id
P0C1Z0
Recombinant Dendroaspis angusticeps Fasciculin-2 (Fas-2)

Recombinant Dendroaspis angusticeps Fasciculin-2 (Fas-2)

$2,466.00 USD