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Recombinant Human Histamine H3 receptor (HRH3), Fluorescent-VLPs

Vendor: CUSABIO

Type: Proteins

SKU: CSB-MP897568HUf4

$6,678.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Human HRH3 protein, Fluorescent-VLPs
Alternative names
(H3R)(HH3R)(G-protein coupled receptor 97)
Buffer [rich text]
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.
Endotoxin
Not test
Expression region
1-445aa
Form
Lyophilized powder
Gene names
HRH3
Mw
75.9 kDa
Notes
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Organism
Homo sapiens (Human)
Protein length
Full Length
Purity
The purity information is not available for VLPs proteins.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Reference [rich text]
"Human skin mast cells express H2 and H4, but not H3 receptors." Lippert U., Artuc M., Grutzkau A., Babina M., Guhl S., Haase I., Blaschke V., Zachmann K., Knosalla M., Middel P., Kruger-Krasagakis S., Henz B.M. J Invest Dermatol 123:116-123(2004)
Relevance
The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 neither modified adenylate cyclase activity nor induced intracellular calcium mobilization.
Research area
Neuroscience
Sds description [rich text]
CSB-MP897568HUf4 is detected by Mouse anti-6*His monoclonal antibody.
Source
Mammalian cell
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
C-terminal EGFP-tagged
Target protein sequenc
MERAPPDGPLNASGALAGEAAAAGGARGFSAAWTAVLAALMALLIVATVLGNALVMLAFVADSSLRTQNNFFLLNLAISDFLVGAFCIPLYVPYVLTGRWTFGRGLCKLWLVVDYLLCTSSAFNIVLISYDRFLSVTRAVSYRAQQGDTRRAVRKMLLVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFFYNWYFLITASTLEFFTPFLSVTFFNLSIYLNIQRRTRLRLDGAREAAGPEPPPEAQPSPPPPPGCWGCWQKGHGEAMPLHRYGVGEAAVGAEAGEATLGGGGGGGSVASPTSSSGSSSRGTERPRSLKRGSKPSASSASLEKRMKMVSQSFTQRFRLSRDRKVAKSLAVIVSIFGLCWAPYTLLMIIRAACHGHCVPDYWYETSFWLLWANSAVNPVLYPLCHHSFRRAFTKLLCPQKLKIQPHSSLEHCWK
Uniprot id
Q9Y5N1
תמונת ברירת מחדל

Recombinant Human Histamine H3 receptor (HRH3), Fluorescent-VLPs

$6,678.00 USD