Recombinant Influenza A virus Nucleoprotein (NP)

Name: Recombinant Influenza A virus Nucleoprotein (NP)
Brand: CUSABIO
Price: 3278.0 USD
Availability: InStock

Vendor: CUSABIO

Type: Proteins

SKU: CSB-BP395556ILQ

$3,278.00 USD

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation

Recombinant Influenza A virus NP protein

Alternative names

Nucleocapsid protein

Buffer [rich text]

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Endotoxin

Not test

Expression region

1-498aa

Form

Liquid or Lyophilized powder

Gene names

NP

Mw

58.9 kDa

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Organism

Influenza A virus (strain A/USA:Albany/12/1951 H1N1)

Protein length

Full Length

Purity

Greater than 85% as determined by SDS-PAGE.

Reconstitution [rich text]

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Relevance

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus

Research area

Others

Sds description [rich text]

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Source

Baculovirus

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Tag info

C-terminal 6xHis-tagged

Target protein sequenc

MASQGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYKRVDRKWMRELVLYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACNSAAFEDLRVSSFIRGTKVIPRGKLSTRGVQIASNENMDTMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDKTTIMAAFTGNAEGRTSDMRAEIIRVMESAKPEEVSFQGRGVFELSDEKAANPIVPSFDMSNEGSYFFGDNAEEYDN

Uniprot id

A4U7B0

Background

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Recombinant Influenza A virus Nucleoprotein (NP)

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