Recombinant Lactococcus lactis subsp. lactis Lantibiotic nisin-Z (nisZ)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP329921LNI

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Lactococcus lactis subsp. lactis nisZ protein
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
24-57aa
Form
Liquid or Lyophilized powder
Gene names
nisZ
Mw
18.8 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Protein length
Full Length of Mature Protein
Purity
Greater than 85% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"Mapping the targeted membrane pore formation mechanism by solution NMR: the nisin Z and lipid II interaction in SDS micelles." Hsu S.T., Breukink E., de Kruijff B., Kaptein R., Bonvin A.M., van Nuland N.A. Biochemistry 41:7670-7676(2002)
Relevance
Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 6xHis-KSI-tagged
Target protein sequenc
ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK
Uniprot id
P29559
Recombinant Lactococcus lactis subsp. lactis Lantibiotic nisin-Z (nisZ)

Recombinant Lactococcus lactis subsp. lactis Lantibiotic nisin-Z (nisZ)

$2,466.00 USD