Recombinant Microbacterium testaceum N-acyl homoserine lactonase (aiiM)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP512020MTN

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Microbacterium testaceum aiiM protein
Alternative names
AHL-lactonase
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
1-251aa
Form
Liquid or Lyophilized powder
Function
Hydrolyzes short- and long-chain N-acyl homoserine lactones with or without 3-oxo substitution at C3. Has slight activity towards L-homoserine lactone, and no activity towards gamma-butyrolactone.
Gene names
aiiM
Mw
43.3 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Microbacterium testaceum (strain StLB037)
Protein length
Full Length
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum."Wang W., Morohoshi T., Ikenoya M., Someya N., Ikeda T.Appl. Environ. Microbiol. 76:2524-2530(2010)
Relevance
Hydrolyzes short- and long-chain N-acyl homoserine lactones with or without 3-oxo substitution at C3. Has slight activity towards L-homoserine lactone, and no activity towards gamma-butyrolactone.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 6xHis-SUMO-tagged
Target protein sequenc
MILAHDVSGSGPLLVLLHGITEDRRSWDPVDFTDGFTVVRVDLRGHGASAAEEPYDIPTLATDVHDTLAQLAENDVIPGELPVIVGHSMGGIVATAYGALFPARAIVNVDQPLQLAGMQGQVQQAEGMLRGADFPLFIHGMFAQMAGGLDAEELARVNGIRSPRQDVVLGMWRPLLEDSPEELAALVSGLTRIPEDVPYLVITGLDAGPEYAAWLQREIPQAVQEVWQPPTHYPHLVDPARFVERVEAFVR
Uniprot id
C6L862
Recombinant Microbacterium testaceum N-acyl homoserine lactonase (aiiM)

Recombinant Microbacterium testaceum N-acyl homoserine lactonase (aiiM)

$2,466.00 USD