Recombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP318842MO

$2,466.00 USD
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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Mouse Lire1 protein
Alternative names
(L1-ORF1p)(LINE retrotransposable element 1)(LINE1 retrotransposable element 1)(Transposase element L1Md-A101/L1Md-A102/L1Md-A2)
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
1-357aa
Form
Liquid or Lyophilized powder
Gene names
Lire1
Mw
48.7 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Mus musculus (Mouse)
Protein length
Full Length
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"Mobilization of LINE-1 retrotransposons is restricted by Tex19.1 in mouse embryonic stem cells." MacLennan M., Garcia-Canadas M., Reichmann J., Khazina E., Wagner G., Playfoot C.J., Salvador-Palomeque C., Mann A.R., Peressini P., Sanchez L., Dobie K., Read D., Hung C.C., Eskeland R., Meehan R.R., Weichenrieder O., Garcia-Perez J.L., Adams I.R. Elife 6:0-0(2017)
Relevance
Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Target protein sequenc
MAKGKRKNPTNRNQDHSPSSERSTPTPPSPGHPNTTENLDPDLKTFLMMMIEDIKKDFHKSLKDLQESTAKELQALKEKQENTAKQVMEMNKTILELKGEVDTIKKTQSEATLEIETLGKRSGTIDASISNRIQEMEERISGAEDSIENIDTTVKENTKCKRILTQNIQVIQDTMRRPNLRIIGIDENEDFQLKGPANIFNKIIEENFPNIKKEMPMIIQEAYRTPNRLDQKRNSSRHIIIRTTNALNKDRILKAVREKGQVTYKGRPIRITPDFSPETMKARRAWTDVIQTLREHKCQPRLLYPAKLSITIDGETKVFHDKTKFTQYLSTNPALQRIITEKKQYKDGNHALEQPRK
Uniprot id
P11260
Recombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1)

Recombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1)

$2,466.00 USD