Recombinant Mycobacterium tuberculosis Transporter PPE51 (PPE51)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP745262GKB

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Mycobacterium tuberculosis PPE51 protein
Alternative names
Proline-proline-glutamate family protein 51;Protein PPE51
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
1-380aa
Form
Liquid or Lyophilized powder
Gene names
PPE51
Mw
44.0 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Protein length
Full Length
Purity
Greater than 85% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Relevance
Small molecule-selective channel required for the uptake of nutrients across the outer mycomembrane. Transports glycerol and glucose. Involved in sensitivity to M.tuberculosis growth inhibitory agrichemical 3,3-bis-di(methylsulfonyl)propionamide (3bMP1). Transports maltose and lactose disaccharides. Involved in sensitivity to bactericidal thio-disaccharide T-6 compound (1,6-anhydro-3-deoxy-4-S-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)-D-glycero-hexopyranos-2-ulose). Transports extracellular trehalose, a component of the cell envelope, and trehalose analog, 6-azido trehalose (6-TreAz), which has antimycobacterial activity.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 6xHis-tagged
Target protein sequenc
MDFALLPPEVNSARMYTGPGAGSLLAAAGGWDSLAAELATTAEAYGSVLSGLAALHWRGPAAESMAVTAAPYIGWLYTTAEKTQQTAIQARAAALAFEQAYAMTLPPPVVAANRIQLLALIATNFFGQNTAAIAATEAQYAEMWAQDAAAMYGYATASAAAALLTPFSPPRQTTNPAGLTAQAAAVSQATDPLSLLIETVTQALQALTIPSFIPEDFTFLDAIFAGYATVGVTQDVESFVAGTIGAESNLGLLNVGDENPAEVTPGDFGIGELVSATSPGGGVSASGAGGAASVGNTVLASVGRANSIGQLSVPPSWAAPSTRPVSALSPAGLTTLPGTDVAEHGMPGVPGVPVAAGRASGVLPRYGVRLTVMAHPPAAG
Uniprot id
P9WHY2
Recombinant Mycobacterium tuberculosis Transporter PPE51 (PPE51)

Recombinant Mycobacterium tuberculosis Transporter PPE51 (PPE51)

$2,466.00 USD