Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)
Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)
Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)

Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP368051MVXc7

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Mycolicibacterium smegmatis ksdD protein
Alternative names
3-keto-Delta(4)-steroid Delta(1)-dehydrogenase;3-oxo-Delta(4)-steroid 1-dehydrogenase
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Expression region
1-569aa
Form
Liquid or Lyophilized powder
Gene names
ksdD
Mw
68.4 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Protein length
Full Length
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Relevance
Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Is also involved in the formation of 1,4-androstadiene-3,17-dione (ADD) from 4-androstene-3,17-dione (AD) to
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
C-terminal 6xHis-tagged
Target protein sequenc
MFYMTGQEYDVVVVGSGAAGMVAALTAAHQGLSTVVVEKAPHYGGSTARSGGGVWIPNNEILKRDGVKDTPDEARKYLHAIIGDVVPAEKIDTYLDRGPEMLSFVLKHSPLKLCWVPGYSDYYPETPGGKPTGRSVEPKPFDANKLGPDLKGLEPPYGKVPMNMVVMQQDYVRLNQLKRHPRGVLRSLKVGIRATWGKVSGKNLVGMGRALIAPLRIGLREAGVPVLLNTALTDLYVEDGRVLGIYVRDTTAGDDAEPRLIRARHGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAVANTGDGIVAAEKLGAALELMEDAWWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGQYGQGPGPGENIPAWLIFDQQYRDRYIFAGLQPGQRIPSKWLESGVIVKADTLVELAEKTGLPADQFTSTIERFNGFARSGVDEDFHRGESAYDRYYGDPTNKPNPNLGEIKHGPFYAAKMVPGDLGTKGGIRTDVRGRALRDDDSVIEGLYAAGNVSSPVMGHTYPGPGGTIGPAMTFGYLAALDIAATAAASRKG
Uniprot id
A0R4S9
Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)

Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)

$2,466.00 USD