Recombinant Rat NAD-dependent protein deacylase sirtuin-5, mitochondrial (Sirt5)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP715034RA

$2,062.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Rat Sirt5 protein
Alternative names
Regulatory protein SIR2 homolog 5;SIR2-like protein 5
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
37-310aa
Form
Liquid or Lyophilized powder
Gene names
Sirt5
Mw
37.2 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Rattus norvegicus (Rat)
Protein length
Full Length of Mature Protein
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Relevance
NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX
Research area
Epigenetics and Nuclear Signaling
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
C-terminal 6xHis-tagged
Target protein sequenc
SSNMADFRKCFANAKHIVIISGAGVSAESGVPTFRGTGGYWRKWQAQHLATPLAFAHNPSQVWEFYHYRREVMRNKEPNPGHLAIAQCEARLRDQGRRVVVITQNIDELHRKAGTKNLLEIHGTLFKTRCTSCGNVAENYKSPICPALLGKGAPEPDTQESRIPVHKLPRCEEAGCGGLLRPHVVWFGENLDPAILKEVDRELARCDLCLVVGTSSVVYPAAMFAPQVASRGVPVAEFNMETTPATNRFRFHFPGPCGVTLPEALAPHETERIS
Uniprot id
Q68FX9
Recombinant Rat NAD-dependent protein deacylase sirtuin-5, mitochondrial (Sirt5)

Recombinant Rat NAD-dependent protein deacylase sirtuin-5, mitochondrial (Sirt5)

$2,062.00 USD