Recombinant Rat VEGF 164
Vendor: ELK Biotechnology
SKU: EPT063
Regular price
$219.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
Product Overview
Product Overview
Accession
P16612-2
Apparent molecular weight
18-23 KDa, reducing conditions
Biological activity
Immobilized Rat VEGF 164(Cat#CJ96) at 2μg/ml (100 μl/well) can bind Mouse VEGFR2-Fc(Cat#C01A).The ED50 of Mouse VEGFR2-Fc(Cat#C01A) is 22.17 ng/ml.
Delivery condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Description
Recombinant Rat Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190(Ala36Thr) is expressed.
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Molecular weight
19.2 KDa
Other names
Vascular endothelial growth factor A; Vascular permeability factor; VEGF; VEGF-A; VPF
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Redissolve
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Background
Vascular endothelial growth factor (VEGF/VEGF-A ) is originally known as vascular permeability factor (VPF). It belongs to the PDGF family with a cysteine-knot structure comprised of eight conserved cysteine residues, and reckoned as a potent mediator in the process of angiogenesis and vasculogenesis in either fetus or adult. VEGF is particularly expressed in supraoptic , paraventricular nuclei and the choroid plexus of the pituitary, and abundant in the corpus luteum of the ovary and in kidney glomeruli. The rat VEGF protein contains a putative 20 amino acids (aa) signal peptide, and alternative splicing of rat VEGF gene produces isoforms of 120, 144, 164 and 188 aa. Rat VEGF164 respectively displays 97% and 88% aa identity with that regions of mouse and human VEGF. VEGF can bind to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin, and play important roles in inducing endothelial cell proliferation, promoting cell migration, inhibiting apoptosis and inducing permeabilization of blood vessels.
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More Info
More Info
Recombinant Rat VEGF 164
Regular price
$219.00 USD
Sale price
Regular price
Unit price
/per

