Recombinant Rhizobium leguminosarum Nitrogenase iron protein (nifH), partial

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP321828RKN

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Rhizobium leguminosarum nifH protein, partial
Alternative names
Nitrogenase Fe protein Nitrogenase component II Nitrogenase reductase
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
1-47aa
Form
Liquid or Lyophilized powder
Function
The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components
Gene names
nifH
Mw
35 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Rhizobium leguminosarum
Protein length
Partial
Purity
Greater than 85% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"The nifH promoter region of Rhizobium leguminosarum: nucleotide sequence and promoter elements controlling activation by NifA protein." Roelvink P.W., Harmsen M., van Kammen A., van den Bos R.C. Gene 87:31-36(1990)
Relevance
The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Target protein sequenc
MAALRQIAFYGKGGIGKSTTSQNTLAALVDHHVPRIPMIIRIGGYAQ
Uniprot id
P20623
Recombinant Rhizobium leguminosarum Nitrogenase iron protein (nifH), partial

Recombinant Rhizobium leguminosarum Nitrogenase iron protein (nifH), partial

$2,466.00 USD