Recombinant Saccharomyces cerevisiae Translation initiation factor IF-2, mitochondrial (IFM1)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-EP335087SVG

$2,466.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant Saccharomyces cerevisiae IFM1 protein
Alternative names
IFM1; YOL023WTranslation initiation factor IF-2; mitochondrial; IF-2(Mt); IF-2Mt; IF2(mt)
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
43-676aa
Form
Liquid or Lyophilized powder
Function
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
Gene names
IFM1
Mw
97.5 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein length
Full Length of Mature Protein
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
Global analysis of protein expression in yeast.Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.Nature 425:737-741(2003)
Relevance
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
Research area
Others
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
E.coli
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal GST-tagged
Target protein sequenc
KGKRRNQISKKELKPLNFSIPNYISVNKLANLLNCRVERLIKDLTALGFENITTTYILSKEYVELILQEYNFALPNLSTSTNLDNVYDELKSPVNPKLLTKRAPVVTIMGHVDHGKTTIIDYLRKSSVVAQEHGGITQHIGAFQITAPKSGKKITFLDTPGHAAFLKMRERGANITDIIVLVVSVEDSLMPQTLEAIKHAKNSGNEMIIAITKIDRIPQPKEREKKIEKVINDLIVQGIPVEKIGGDVQVIPISAKTGENMDLLEESIVLLSEVMDIRAENSPKTIAEGWIIESQVKKQVGNVATVLVKKGTLQKGKILICGNTFCKIKNLIDDKGIPILKATPSYATEVLGWKDVPHVGDEVIQVKSEAIAKKFISKRQDLIEVQKNSSIVEKLNEERALAKEQHLNKELEHENTVQEHEQNTGPKLINYIIKCDVSGSAEAVSESISSLGNDEVRCNVISSSVGIPTESDLKMAQITESTILCFNLGNLPSEVINNRAGIKIKQYNVIYKLIEDVTETLTENLKPIFEKKIVSTVDVRETFDFRLKKKIIRIAGCKVNNGVIKKNSLVQVVRGPNEDVIFDGKISTLKHNKDDVAEVSKGHECGITFESGFEGFKPGDKILVYENVRVPRYL
Uniprot id
P25038
Recombinant Saccharomyces cerevisiae Translation initiation factor IF-2, mitochondrial (IFM1)

Recombinant Saccharomyces cerevisiae Translation initiation factor IF-2, mitochondrial (IFM1)

$2,466.00 USD