Recombinant Schizosaccharomyces pombe NADH kinase pos5, mitochondrial (pos5)
Recombinant Schizosaccharomyces pombe NADH kinase pos5, mitochondrial (pos5)

Recombinant Schizosaccharomyces pombe NADH kinase pos5, mitochondrial (pos5)

Vendor: CUSABIO

Type: Proteins

SKU: CSB-YP891853SXV

$2,959.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Abbreviation
Recombinant SchizosaccharoMyces pombe pos5 protein
Alternative names
pos5; SPAC323.01c; NADH kinase pos5; mitochondrial; EC 2.7.1.86
Buffer [rich text]
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Endotoxin
Not test
Expression region
43-386aa
Form
Liquid or Lyophilized powder
Function
Phosphorylates both NADH and NAD(+), with a preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH (By similarity).
Gene names
pos5
Mw
40.7 kDa
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Protein length
Full Length of Mature Protein
Purity
Greater than 90% as determined by SDS-PAGE.
Reconstitution [rich text]
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Reference [rich text]
"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.Nat. Biotechnol. 24:841-847(2006)
Relevance
Phosphorylates both NADH and NAD+, with a preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH
Research area
Microbiology
Sds description [rich text]
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source
Yeast
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Tag info
N-terminal 6xHis-tagged
Target protein sequenc
NSVYSKSIRLVNTLENRIVPVYKECASPQSIGGKSNLKQLQWPKPPKNILILKKRMDERVDHCFETLVQHLQQTYPDICIITETDVAKKFSYLNLYTWTEISDLEQKVDAIITVGGDGTILHAASLFARSGMPPILSFSLGTLGFLLPFDFGSFQTAFADFYNSRSFVLMRMRLRVAMKTKLYNESIYAMNEMHIHRGLSPHMAVLKVFVNDKFLTEAVADGLIISTPTGSTAYSLSSGGPIVHPSINALLLTPICPNSLSFRPVLFPDTFKISIETSNKSRVRPQLSIDGRPLGLTDIGQRIDITSVKDNAIPCIIRSHKEDDWVSDIVSLLRWNHPFHRKGW
Uniprot id
Q9UT98
Recombinant Schizosaccharomyces pombe NADH kinase pos5, mitochondrial (pos5)

Recombinant Schizosaccharomyces pombe NADH kinase pos5, mitochondrial (pos5)

$2,959.00 USD