Sheep IL1a(Interleukin 1 Alpha) ELISA Kit
Sheep IL1a(Interleukin 1 Alpha) ELISA Kit

Sheep IL1a(Interleukin 1 Alpha) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8098

$412.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
15.63-1000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Sheep
Research area
Cytokine;Apoptosis;
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity
6 pg/mL
Standard
1000 pg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IL1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep IL1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep IL1a in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q28579
Sheep IL1a(Interleukin 1 Alpha) ELISA Kit

Sheep IL1a(Interleukin 1 Alpha) ELISA Kit

$412.00 USD