Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit
Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit
Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit
Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit

Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK0009

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$458.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit

Sheep sIgA(Secretory Immunoglobulin A) ELISA Kit

$458.00 USD
$0.00 USD

THE SCIENCE, IN DETAIL

Know your product.

Specifications
Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 µg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Sheep
Research area
Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;
Sample type
serum, plasma, saliva and other biological fluids
Sensitivity
0.049 µg/mL
Standard
10 µg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep sIgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep sIgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep sIgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep sIgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical documents

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