The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

P50414

around 3 working days

L 16cm x W 15cm x H 9cm

Cytokine;Tumor immunity;Infection immunity;

3.5h

serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids

31.25-2000 pg/mL

2000 pg/mL

12.1 pg/mL

Sandwich

Sheep