Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat heart tissue, Mouse brain tissu; All lanes: SOD2 antibody at 3.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 25, 21, 19, 20 kDa; Observed band size: 25 kDa
IHC image diluted at 1:560 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC07010 at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating SOD2 in K562 whole cell lysate; , Lane 1: Rabbit control IgG instead in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC07010 (8µg) + K562 whole cell lysate (500µg); , Lane 3: K562 whole cell lysate (10µg)
Sod2 Polyclonal Antibody - image 5
Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat heart tissue, Mouse brain tissu; All lanes: SOD2 antibody at 3.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 25, 21, 19, 20 kDa; Observed band size: 25 kDa
IHC image diluted at 1:560 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC07010 at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating SOD2 in K562 whole cell lysate; , Lane 1: Rabbit control IgG instead in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC07010 (8µg) + K562 whole cell lysate (500µg); , Lane 3: K562 whole cell lysate (10µg)
Sod2 Polyclonal Antibody - image 5

Sod2 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC07010-100UG

$510.25 USD
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SDS

Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P04179
Applications
ELISA, WB, IHC, IF, IP
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Sod2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Superoxide Dismutase [Mn], Mitochondrial Protein (64-175Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
SOD2
Target synonyms
Indophenoloxidase B; IPO B; IPOB; Manganese containing superoxide dismutase; Manganese SOD; Manganese superoxide dismutase; Mangano superoxide dismutase; Mn SOD; Mn superoxide dismutase; MNSOD; MVCD6; SOD 2; SOD2; SODM_HUMAN; Superoxide dismutase [Mn] mitochondrial; Superoxide dismutase [Mn]; mitochondrial; Superoxide dismutase 2 mitochondrial
Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat heart tissue, Mouse brain tissu; All lanes: SOD2 antibody at 3.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 25, 21, 19, 20 kDa; Observed band size: 25 kDa

Sod2 Polyclonal Antibody

$510.25 USD