Endotoxin-Free Plasmid Extraction Mini Kit | SPICA
SPICA Endotoxin-Free Plasmid Extraction Mini Kit provides a streamlined workflow for isolating high-purity plasmid DNA from bacterial cultures. The product is configured for clear, repeatable laboratory operation and compatibility with the specified...
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Product overview
SPICA Endotoxin-Free Plasmid Extraction Mini Kit provides a streamlined workflow for isolating high-purity plasmid DNA from bacterial cultures. The product is configured for clear, repeatable laboratory operation and compatibility with the specified sample format.
Product highlights
- Format/Principle: Mini Spin Column (silica matrix)
- Sample Size: 1~5 ml of bacterial culture for high/low-copy number plasmids.
- Size of Plasmid or Construct: <15 kbp
- Operation Time: <30 mins
- Typical Yield: 25~40 µg
- Column Capacity: ≤60 µg DNA/column
- Column Applicability: Centrifugation and vacuum
Recommended applications
PCR, qPCR, cloning, sequencing and library preparation, subject to the selected sample type and protocol.
Components and storage
| Component | Storage |
|---|---|
| PM1 Buffer | Stored at room temperature between 15~ 25 °C . |
| PM2 Buffer | Stored at room temperature between 15~ 25 °C . |
| PM3 Buffer | Stored at room temperature between 15~ 25 °C . |
| FAER Buffer | Stored at room temperature between 15~ 25 °C . |
| WP Buffer | Stored at room temperature between 15~ 25 °C . |
| Wash Buffer (Concentrate) | Stored at room temperature between 15~ 25 °C . |
| Elution Buffer | Stored at room temperature between 15~ 25 °C . |
| FAPD Mini Columns | Stored at room temperature between 15~ 25 °C . |
| Collection Tubes | Stored at room temperature between 15~ 25 °C . |
| Elution Tubes | Stored at room temperature between 15~ 25 °C . |
| RNase A Solution | Store at -20 °C upon receipt. |
SPICA Endotoxin-Free Plasmid Extraction Mini Kit provides a streamlined workflow for isolating high-purity plasmid DNA from bacterial cultures, with a practical format for…
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