T4 Polynucleotide Kinase

T4 Polynucleotide Kinase catalyzes the transfer of the Y-phosphate of ATP to the 5'-hydroxyl-terminus of DNA or RNA, or the 5'- phosphoterminus of DNA or RNA using the exchange reaction. This makes T4 Polynucleotide Kinase suitable for 5'-end-labeling of double-stranded DNA and 5'-end-labeling of oligonucleotides or RNA. T4 Polynucleotide Kinase is supplied in 50 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 µM ATP, and 50% glycerol. T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate of ATP to the 5'-OH termini of polynucleotides. This reaction is reversible. In the presence of ATP, 5'-OH termini are phosphorylated (phosphorylation reaction), and in the presence of ADP, 5'-P termini are dephospharylated. By adding [γ-32P] ATP to the reaction mixture, the phosphorylation reaction occurs simultaneously with the exchange of the 5'-P termini with labeled phosphate (the phosphate exchange reaction). Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl2 and 50 mM DTT]. One unit is defined as the amount of enzyme that incorporates 1 nmol of [32P] from [Y-32P]ATP into acid-insoluble products in 30 minutes at 37°C and pH 7.6, using calf thymus DNA activated by micrococcal nuclease as the substrate.