Thymidine Auxotrophic EHA105 Agrobacterium Chemically Competent Cells

Intact Genomics (ig®) Thymidine Auxotrophic EHA105Thy Agrobacterium Chemically Competent Cells include modifications so that they will not grow unless 50 mg/L of thymidine is added to Minimal medium. This prevents the bacteria from overgrowing plant tissues when used for plant transformation. Thymidine-Auxotrophic EHA105Thy Agrobacterium Chemically Competent Cells are optimized for the highest transformation efficiencies. The EHA105Thy strain is useful for transgenic operations of rice, tobacco and other plants. EAH105Thy– thyA knockout mutant derived from EHA105, itself a derivative of A281 (A136/pTiBo542). Benefits: Thymidine Auxotrophic Enables development of more efficient transformation systems Reduced bacterial overgrowth during co-cultivation Decreased need for antibiotics Product Components: ig® Thymidine-Auxotrophic EHA105Thy Agrobacterium Chemically Competent Cells DNA (pCAMBIA1391z, 500 pg/µl) Recovery medium* * Client needs to add 50 mg/L of thymidine (not provided) to recovery media before use